Pituitary, normal, high power

Before the advent of the immunohistochemical stains and electron microscopy, it was only possible to stain for three cell types in the pituitary. Those were the basophilic cells, the eosinophilic cells and the chromophobic cells (e.g., H & E stain as shown here).

This specimen is a section of the anterior pituitary. Notice that some of the nuclei are surrounded by cytoplasm which has stained pink, some of the nuclei have cytoplasm which has stained blue and some of the nuclei have cytoplasm which has not taken up any stain. At the present time immunohistochemical stains are available which allow for the specific staining of the different cells in the pituitary, using antibodies directed against the hormone of interest.